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Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro ArchiMer
Hellio, Claire; Bado Nilles, Anne; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Helene.
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at...
Tipo: Text Palavras-chave: Prophenoloxidase; Phenoloxidase; Oyster; Immunity; Defence; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2926.pdf
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Effets de pollutions par hydrocarbures sur les capacités de défense d'organismes marins ArchiMer
Bado Nilles, Anne.
The effects of PAHs, considered among the most toxic by the United States Environmental Protection Agency, were tested in vitro and in vivo on two commercial species of the Pertuis-Charentais (Charente-Maritime, France): sea bass, Dicentrarchus labrax, and the Pacific oyster, Crassostrea gigas. This study was carried out as part of the European project EROCIPS with the aim of finding new immunological biomarkers caused by occasional pollution by hydrocarbons. During in vitro experimentation, pollutants and immunological biomarkers were choosen. Thereafter, the in vitro exposures to the soluble fraction of Erika's heavy fuel oil and its fluxant, light cycle oil, began. These exposures enable the validation of the experimental system used, with, in...
Tipo: Text Palavras-chave: Biliary metabolites; Bioaccumulation; Toxicity; Immune défense; European sea bass; Pacific oyster; Métabolites biliaires; Bioaccumulation; Toxicité; Capacités de défense; Hydrocarbure; Bar commun; Huître creuse.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-7401.pdf
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